本品是在大肠杆菌BL21(DE3)菌株基础上制备的化学转化感受态细胞,是以T7 RNA聚合酶为表达系统的高效蛋白表达宿主。可同时表达T7 RNA聚合酶和大肠杆菌RNA聚合酶,用于pET系列,pGEX,pMAL等质粒的蛋白表达。适合于非病毒蛋白重组表达。使用pUC19检测转化效率达107 cfu/μg DNA以上。
BL21(DE3)感受态细胞基因型:F-ompT hsdSB (rB-mB-) gal dcm (DE3)。
- 适合于非病毒蛋白重组表达。
-85~-65℃保存,有效期6个月。请勿将本品置于-20℃或液氮中保存。
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