Hieff NGS® DNA Selection Beads基于SPRI (Solid Phase Reverse Immobilization)原理，配合精心优化过的缓冲体系，可用于二代测序文库构建过程中的DNA片段分选、纯化。本产品可适用于各品牌的DNA、RNA建库试剂盒，和目前广泛使用的AMPure XP Beads使用方式相同，片段回收效率和文库大小分布均与AMPure XP Beads高度吻合。
品质保证：采用进口原材料，品质高度稳定。
无缝替换：产品原理、产品操作和实验结果与AMPure XP保持高度一致。
兼容性高：兼容目前市面上所有的DNA/RNA建库试剂盒。
批次稳定：严格的批次性能与稳定性质控。
- 高效的纯化回收效率
DNA磁珠纯化过程中会有部分样本损失，回收效率可通过公式计算：回收效率 =（纯化后DNA质量 / Input DNA质量）× 100%。数据显示，12601磁珠批次稳定性良好，回收效率与XP磁珠相当，是高性价比的替代产品。
2~8℃保存，有效期18个月。
Q：12601可以用来纯化DNA吗？
A：可以纯化150bp以上的DNA片段，如果纯化的DNA中含有较多的150bp以下的建议用货号12600的产品，可以纯化50bp以上的DNA。
Q：12601可以分选1Kb或以上的DNA片段吗？
A：需要自行摸索体系，目前筛选的片段范围确定的体系为说明中的体系，其它片段分选的可以参考说明书中的分选体系调整。
Q： 不小心将磁珠放到-20℃，但未结冰，是否可以继续使用？
A：不建议使用。因为低温会破坏磁珠的结构，使得磁珠的抓取效率降低，影响磁珠回收性能。
Q：磁珠未开封的时候 4℃保存，开封后一直室温保存使用，是否会影响磁珠的回收性能？
A：室温放置对磁珠的回收性能会有一定影响，不建议室温放置太久，使用完后建议 4℃ 保存。PEG 分离效果易受 pH、温度等影响。
Q：磁珠回收率低可能的原因是什么？
A：1）磁珠与DNA 混匀不充分，未能充分吸附。建议反应体系充分混匀；
2）磁珠比例不对，建议按照说明书进行选择合适的比例；
3）孵育时间不足，保证孵育时间至少 5 min；
4）磁珠分选时，二轮磁珠吸附的时候吸到磁珠。可在 200 μL 枪头前串联 10μL 枪头用于移液，可防止吸到磁珠；
5）磁珠洗涤时的乙醇非现配，导致乙醇浓度过低，建议乙醇浓度不低于 70%；
6）干燥过度：磁珠长时间干燥导致表面龟裂，DNA 难以洗脱，得率降低；建议干燥时间不宜过长，表面刚出现龟裂即可；
7）洗脱液体积不足：最终洗脱液应完全覆盖管内磁珠。
Q：12601可以纯化或回收150bp以下的片段吗？
A：不建议使用12601，建议使用12600，最低可回收100bp左右的片段，50bp以上的回收率可能会有影响。
Q：是否可以纯化单链DNA，该使用多少磁珠？
A：磁珠可以吸附双链DNA和单链DNA，但不能区分单链或双链DNA。磁珠纯化单链DNA的倍数可以参考双链的说明书。
[1]He J, Liu T, Wang W, Wu X, Wang J, Yan W. Comprehensive improvement of soil quality and rice yield by flooding-midseason drying-flooding. Appl Microbiol Biotechnol. 2022;106(21):7347-7359. doi:10.1007/s00253-022-12184-7(IF:5.560)
[2]Wang X, Yuan Q, Zhang W, et al. Sequence specific integration by the family 1 casposase from Candidatus Nitrosopumilus koreensis AR1. Nucleic Acids Res. 2021;49(17):9938-9952. doi:10.1093/nar/gkab725(IF:19.160)
[3]Duan XZ, Sun JT, Wang LT, et al. Recent infection by Wolbachia alters microbial communities in wild Laodelphax striatellus populations. Microbiome. 2020;8(1):104. Published 2020 Jul 2. doi:10.1186/s40168-020-00878-x(IF:16.837)
[4]Ghosh S, Yang X, Wang L, Zhang C, Zhao L. Active phase prebiotic feeding alters gut microbiota, induces weight-independent alleviation of hepatic steatosis and serum cholesterol in high-fat diet-fed mice. Comput Struct Biotechnol J. 2020;19:448-458. Published 2020 Dec 24. doi:10.1016/j.csbj.2020.12.011(IF:6.155)
[5]Gao X, Yu B, Yu J, et al. Developmental Profiling of Dietary Carbohydrate Digestion in Piglets. Front Microbiol. 2022;13:896660. Published 2022 Apr 29. doi:10.3389/fmicb.2022.896660(IF:6.064)
[6]Li P, Zhang Y, Yan F, Zhou X. Characteristics of a Bacteriophage, vB_Kox_ZX8, Isolated From Clinical Klebsiella oxytoca and Its Therapeutic Effect on Mice Bacteremia. Front Microbiol. 2021;12:763136. Published 2021 Dec 3. doi:10.3389/fmicb.2021.763136(IF:6.064)
[7]Lin Z, Luo P, Huang D, Wu Y, Li F, Liu H. Multi-omics based strategy for toxicity analysis of acrylamide in Saccharomyces cerevisiae model. Chem Biol Interact. 2021;349:109682. doi:10.1016/j.cbi.2021.109682(IF:5.168)
[8]Sun X, Lv W, Wang Y, et al. Mrgprb2 gene plays a role in the anaphylactoid reactions induced by Houttuynia cordata injection. J Ethnopharmacol. 2022;289:115053. doi:10.1016/j.jep.2022.115053(IF:5.195)
[9]Ma H, Lai B, Zan C, Di X, Zhu X, Wang K. GLO1 Contributes to the Drug Resistance of Escherichia coli Through Inducing PER Type of Extended-Spectrum β-Lactamases. Infect Drug Resist. 2022;15:1573-1586. Published 2022 Apr 5. doi:10.2147/IDR.S358578(IF:4.177)
[10]Zhong Y, Zhao W, Tang Z, et al. Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii. BMC Genomics. 2021;22(1):199. Published 2021 Mar 21. doi:10.1186/s12864-021-07537-x(IF:4.547)
[11]Lian C, Yang H, Lan J, et al. Comparative analysis of chloroplast genomes reveals phylogenetic relationships and intraspecific variation in the medicinal plant Isodon rubescens. PLoS One. 2022;17(4):e0266546. Published 2022 Apr 6. doi:10.1371/journal.pone.0266546(IF:3.752)
[12]Diao G, Huang J, Zheng X, et al. Prostaglandin E2 serves a dual role in regulating the migration of dendritic cells. Int J Mol Med. 2021;47(1):207-218. doi:10.3892/ijmm.2020.4801(IF:5.314)
[13]Bing XL, Zhao DS, Peng CW, Huang HJ, Hong XY. Similarities and spatial variations of bacterial and fungal communities in field rice planthopper (Hemiptera: Delphacidae) populations. Insect Sci. 2020;27(5):947-963. doi:10.1111/1744-7917.12782(IF:3.605)
[14]Li X, Zhou S, Zhang J, Zhou Z, Xiong Q. Directional Changes in the Intestinal Bacterial Community in Black Soldier Fly (Hermetia illucens) Larvae. Animals (Basel). 2021;11(12):3475. Published 2021 Dec 6. doi:10.3390/ani11123475(IF:3.231)
[15]Yang J, Peng Y, Kong W. Identification and complete genome sequence of mulberry cryptic virus 1. Arch Virol. 2022;167(2):687-690. doi:10.1007/s00705-021-05350-1(IF:2.685)
[16]Liu S, Luorong Q, Hu K, et al. Aqueous Extract of Lysimachia christinae Hance Prevents Cholesterol Gallstone in Mice by Affecting the Intestinal Microflora. J Microbiol Biotechnol. 2021;31(9):1272-1280. doi:10.4014/jmb.2106.06043(IF:3.277)
[17]Chang Y, Xia X, Sui L, et al. Endophytic colonization of entomopathogenic fungi increases plant disease resistance by changing the endophytic bacterial community. J Basic Microbiol. 2021;61(12):1098-1112. doi:10.1002/jobm.202100494(IF:2.650)
[18]Ding CY, Ma YM, Li B, et al. Identification and Functional Analysis of Differentially Expressed Genes in Myzus persicae (Hemiptera: Aphididae) in Response to Trans-anethole. J Insect Sci. 2022;22(1):3. doi:10.1093/jisesa/ieab094(IF:2.066)
[19]Liu QH, Wang ZY, Tang JW, et al. Comparative transcriptome analysis of diurnal alterations of liver glycogen structure: A pilot study. Carbohydr Polym. 2022;295:119710. doi:10.1016/j.carbpol.2022.119710(IF:10.723)
[20]Huang C, Mei Q, Lou L, et al. Ulcerative Colitis in Response to Fecal Microbiota Transplantation via Modulation of Gut Microbiota and Th17/Treg Cell Balance. Cells. 2022;11(11):1851. Published 2022 Jun 5. doi:10.3390/cells11111851(IF:7.666)
[21]Jing F, YR Zheng, Xin Z,et al, Effect of supercritical carbon dioxide on bacterial community, volatile profiles and quality changes during storage of Mongolian cheese, Food Control, Volume 143,2023,https://doi.org/10.1016/j.foodcont.2022.109225(IF:6.652)
[22]Huang C, Gao F, Zhou H, et al. Oral Microbiota Profile in a Group of Anti-AChR Antibody-Positive Myasthenia Gravis Patients. Front Neurol. 2022;13:938360. Published 2022 Jul 7. doi:10.3389/fneur.2022.938360(IF:4.086)
