本品是由Cy3标记的山羊抗兔IgG(H+L),使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子,也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应,但不会识别非免疫球蛋白类的血清蛋白。
Cy3最大激发波长(Amax)为550 nm,最大发射波长(Emax)为570 nm。本品适合做单标实验。要做多标(multiple-labeling)实验,建议使用与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。
抗体浓度(Antibody Concentration) |
100 µL(0.75 mg/mL) |
缓冲液(Buffer) |
0.005 M磷酸钠,0.125 M氯化钠,pH 7.6 |
稳定剂(Stabilizer) |
7.5 mg/mL BSA(无IgG,蛋白酶),50%甘油 |
防腐剂(Preservative) |
0.025% 叠氮化钠 |
原料来源(Source of Material) |
Jackson Immunoresearch 111-165-003 |
冰袋运输。-20℃分装保存,尽量避免反复冻融。有效期1年。
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