Dulbecco的改良Eagle培养基—DMEM（Dulbecco's Modified Eagle Medium）是一种广泛使用的基础培养基，适用于多种哺乳动物细胞培养，包括原代成纤维细胞，神经元，神经胶质细胞，HUVEC和平滑肌细胞，以及HeLa，293，Cos-7和PC-12等细胞系。DMEM是在MEM培养基的基础上研制的，与MEM培养基相比，氨基酸的含量增加了2倍，维生素增加了4倍，同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。
DMEM培养基最初设计为葡萄糖含量1000 mg/L的低糖型，后来又发展出葡萄糖含量为4500 mg/L的高糖型，现已广泛应用于各种细胞的培养。DMEM高糖型普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、原代病毒宿主细胞、单一细胞的培养以及疫苗的生产，例如利用CHO细胞表达EPO和生产乙肝疫苗。
L-谷氨酰胺是细胞培养液体环境中所必需的一种营养成分，但其在水溶液中不稳定，易降解。丙酮酸钠可以作为细胞培养中的替代碳源，在葡萄糖不足的情况下，细胞也可以代谢丙酮酸钠。
本产品含有：D-葡萄糖(4500 mg/L)、酚红、L-谷氨酰胺、丙酮酸钠。
不含有：HEPES、双抗。
产品成分
| Components | Molecular Weight | Concentration (mg/L) | mM |
| Amino Acids | |||
| Glycine | 75.0 | 30.0 | 0.4 |
| L-Arginine hydrochloride | 211.0 | 84.0 | 0.39810428 |
| L-Cystine 2HCl | 313.0 | 63.0 | 0.20127796 |
| L-Glutamine | 146.0 | 584.0 | 4.0 |
| L-Histidine hydrochloride-H2O | 210.0 | 42.0 | 0.2 |
| L-Isoleucine | 131.0 | 105.0 | 0.8015267 |
| L-Leucine | 131.0 | 105.0 | 0.8015267 |
| L-Lysine hydrochloride | 183.0 | 146.0 | 0.7978142 |
| L-Methionine | 149.0 | 30.0 | 0.20134228 |
| L-Phenylalanine | 165.0 | 66.0 | 0.4 |
| L-Serine | 105.0 | 42.0 | 0.4 |
| L-Threonine | 119.0 | 95.0 | 0.79831934 |
| L-Tryptophan | 204.0 | 16.0 | 0.078431375 |
| L-Tyrosine disodium salt dihydrate | 261.0 | 104.0 | 0.39846742 |
| L-Valine | 117.0 | 94.0 | 0.8034188 |
| Vitamins | |||
| Choline chloride | 140.0 | 4.0 | 0.028571429 |
| D-Calcium pantothenate | 477.0 | 4.0 | 0.008385744 |
| Folic Acid | 441.0 | 4.0 | 0.009070295 |
| Niacinamide | 122.0 | 4.0 | 0.032786883 |
| Pyridoxine hydrochloride | 206.0 | 4.0 | 0.019417476 |
| Riboflavin | 376.0 | 0.4 | 0.0010638298 |
| Thiamine hydrochloride | 337.0 | 4.0 | 0.011869436 |
| i-Inositol | 180.0 | 7.2 | 0.04 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111.0 | 200.0 | 1.8018018 |
| Ferric Nitrate (Fe(NO3)3-9H2O) | 404.0 | 0.1 | 2.4752476E-4 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120.0 | 97.67 | 0.8139166 |
| Potassium Chloride (KCl) | 75.0 | 400.0 | 5.3333335 |
| Sodium Chloride (NaCl) | 58.0 | 6400.0 | 110.344826 |
| Sodium Phosphate monobasic (NaH2PO4-H2O) | 138.0 | 125.0 | 0.9057971 |
| Sodium Bicarbonate (NaHCO3) | 84.0 | 3700.0 | 44.0 |
| Other Components | |||
| D-Glucose (Dextrose) | 180.0 | 4500.0 | 25.0 |
| Phenol Red | 376.4 | 15.0 | 0.039851222 |
| Sodium Pyruvate | 110.0 | 110.0 | 1.0 |
2-8℃避光保存，一年有效。
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