乳酸脱氢酶细胞毒性检测试剂盒(LDH Cytotoxicity Assay Kit)，也称乳酸脱氢酶检测试剂盒(LDH Assay Kit)或乳酸脱氢酶释放检测试剂盒(LDH Release Assay Kit)，是一种基于diaphorase催化的INT显色反应，通过比色法检测细胞毒性时释放的乳酸脱氢酶活性或检测其它样品中的乳酸脱氢酶活性的试剂盒。细胞凋亡或坏死而造成的细胞膜结构的破坏会导致细胞浆内的酶释放到培养液里，其中包括酶活性较为稳定的乳酸脱氢酶(lactate dehydrogenase, LDH)。通过检测从质膜破裂的细胞中释放到培养液中的LDH的活性，就可以实现对细胞毒性的定量分析。LDH释放被看做细胞膜完整性的重要指标，并被广泛用于细胞毒性检测。LDH释放被认为是以前使用放射性的51Cr标记细胞，随后通过51Cr释放进行细胞膜完整性检测的安全有效的替代方法。本试剂盒的基本原理是，在乳酸脱氢酶的作用下，NAD+被还原生成NADH，NADH和INT (2-p-iodophenyl-3-nitrophenyl tetrazolium chloride)被硫辛酰胺脱氢酶(diaphorase)催化反应生成NAD+和强生色物甲臜(formazan)，在490 nm波长下产生吸收峰，从而通过比色来定量检测细胞培养液、细胞裂解液等样品中乳酸脱氢酶的活性。吸光度与乳酸脱氢酶活性成线性正相关。该酶联反应原理的示意图如下:
即用、便捷。
-25~-15℃保存，避光保存，有效期1年。
Q:血清会影响检测结果吗？
A:由于血清含有乳酸脱氢酶，建议血清的使用浓度不要超过 1%，并最好使用热灭活血清。
Q:收取细胞上清液中释放的 LDH，在冰箱放置过夜，第二天检测可以吗？
A:收取细胞上清液中释放的 LDH，可以在冰箱放置过夜，第二天检测。但是为了减少实验误差，建议客户最好是现取现测。
Q: 乳酸脱氢酶细胞毒性检测试剂盒，对照的死亡率怎么算？
A: 这个试剂盒一般是计算样品的死亡率, 不计算对照的死亡率呀，而且样品的死亡率也要用对照的来计算。
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