Super ECL Detection Reagent ECL化学发光超敏显色试剂盒
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36208ES60
100 mL
现货
¥ 378.00
36208ES76
500mL
现货
¥ 1578.00
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产品介绍
ECL化学发光超敏显色试剂盒用于检测直接或间接标记辣根过氧化物酶(HRP)的抗体及其关联的抗原。其原理是,蛋白质或核酸在电泳后转移到印迹膜上,以一抗及HRP标记的二抗结合膜上的目的蛋白,或以HRP标记的探针直接或间接结合膜上的核酸。洗膜后用本产品配制的ECL工作液,室温孵育膜数分钟,将印迹膜用保鲜膜包被粘贴固定于X光片曝光暗盒。然后转入暗室将X光胶片压在膜上曝光数秒到数小时,显影定影后蛋白质或核酸条带可清晰显示在X光胶片上。
本试剂盒采用了独特的发光底物系统,ECL化学发光超敏显色试剂盒是目前最灵敏的商业化荧光ECL检测试剂。
翌圣为您提供Western Blot实验整体解决方案,相关产品选购请参考:WB实验系列产品-选购指南
产品性质
产品特色
1.具有极高灵敏度和高信噪比,可检测低皮克级抗原;
2.发光迅速,亮度强,可在日光灯下观察到印迹膜条带;
3.持续发光时间长,荧光可使X光胶片感光达5小时以上,特别适用于痕量蛋白或核酸检测;
4.可使用更高的抗体稀释倍数(1∶2000~1∶10000),大大节省抗体。
存储条件
2~8℃避光保存。有效期1年。
FAQ
Q:该产品可以检测低皮克级的抗原吗?
A:可以的,本品为超敏型试剂盒。
Q:使用该产品的一抗浓度是多少?
A:1:1000-1:10000。
Q:该产品能适用于 PVDF 膜吗?
A:可以的,NC 膜或PVDF 膜均适用。
Q:若样本靶点丰度较高,可以使用该产品吗?
A:不推荐,该品适用于靶点丰度低的样本。
产品文档
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已发表文献
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