Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

稀释比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/50-1/200

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产品数据
Western blot

Western blot analysis of Smad3 in Jurkat, C6, HeLa lysates using Smad3 antibody.

Immunohistochemistry

Immunocytochemistry analysis of Smad3(green) in A549 using Smad3 antibody, and DAPI(blue).

Immunohistochemistry

Immunocytochemistry analysis of paraffin-embedded Human breast cancer tissue using Smad3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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