ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.

稀释比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/50-1/200; IP:1/20;

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产品数据

Western blot

Western blot analysis of Estrogen Receptor alpha in K562, HeLa lysates using Estrogen Receptor alpha antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Estrogen Receptor alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry

Immunocytochemistry analysis of Estrogen Receptor alpha(green) in MCF-7 using Estrogen Receptor alpha antibody, and DAPI(blue).

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