Hieff® Taq DNA Polymerase DNA聚合酶®
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10101ES80
1000U
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¥ 128.00
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10101ES92
10000U
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Hieff® Taq DNA Polymerase是嗜热性Thermus aquaticus表达的热稳定重组型DNA聚合酶,分子量为94 KD。具有5’→3’聚合酶活性和5’→3’外切酶活性,无3’→5’外切酶活性。扩增产物具有3’-dA,可直接用于TA克隆。

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1、活性定义:用活性化的大马哈鱼精子DNA作为模板/引物,74℃,30 min内,摄入10 nmol的全核苷酸为酸性不溶物的活性定义为1个活性单位(U)。

2、适用于基因型鉴定、菌落PCR等常规PCR。


存储条件

-25~-15℃保存,有效期2年。

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已发表文献

[1] Lu Z, Yang S, Yuan X, et al. CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis. Nucleic Acids Res. 2019;47(7):e40. doi:10.1093/nar/gkz072(IF:11.147)

[2] Chen X, Liang H, Xi Z, et al. BM-MSC Transplantation Alleviates Intracerebral Hemorrhage-Induced Brain Injury, Promotes Astrocytes Vimentin Expression, and Enhances Astrocytes Antioxidation via the Cx43/Nrf2/HO-1 Axis. Front Cell Dev Biol. 2020;8:302. Published 2020 May 8. doi:10.3389/fcell.2020.00302(IF:5.186)

[3] Fu C, Xu J, Jia Z, Yao K, Chen X. Cataract-causing mutations L45P and Y46D promote γC-crystallin aggregation by disturbing hydrogen bonds network in the second Greek key motif. Int J Biol Macromol. 2021;167:470-478. doi:10.1016/j.ijbiomac.2020.11.158(IF:5.162)

[4] Chen X, Xu CX, Liang H, et al. Bone marrow mesenchymal stem cells transplantation alleviates brain injury after intracerebral hemorrhage in mice through the Hippo signaling pathway. Aging (Albany NY). 2020;12(7):6306-6323. doi:10.18632/aging.103025(IF:4.831)

[5] Fu C, Xu J, Yang X, Chen X, Yao K. Cataract-causing mutations L45P and Y46D impair the thermal stability of γC-crystallin. Biochem Biophys Res Commun. 2021;539:70-76. doi:10.1016/j.bbrc.2020.12.096(IF:3.575)

[6] Fei ZY, Wang WS, Li SF, et al. High expression of the TEFM gene predicts poor prognosis in hepatocellular carcinoma. J Gastrointest Oncol. 2020;11(6):1291-1304. doi:10.21037/jgo-20-120(IF:2.536)

[7] Zhang L, Li Y, Bao H, et al. Population structure and antimicrobial profile of Staphylococcus aureus strains associated with bovine mastitis in China. Microb Pathog. 2016;97:103-109. doi:10.1016/j.micpath.2016.06.005(IF:1.888)

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