苯甲基磺酰氟（PMSF）是一种不可逆的丝氨酸蛋白酶抑制剂，如胰凝乳蛋白酶、胰蛋白酶、凝血酶等，、作用原理是特异性识别并磺化决定酶活性的丝氨酸残基活性位点。此外，其对半胱氨酸蛋白酶（如木瓜蛋白酶，DTT逆转其抑制活性）和哺乳动物来源的乙酰胆碱酯酶也有抑制效果。PMSF常在细胞或组织裂解之前加入到裂解液中以预防蛋白的降解。PMSF可以单独使用，也常与其他蛋白酶抑制剂如胃抑肽酶A或者磷酸酶抑制剂如正钒酸钠混合使用。
本品以粉末形式提供，难溶于水，且在水溶液中的稳定性很差，必须在使用前才加入裂解液或其他水溶液中。PMSF粉末可用甲醇，乙醇或者异丙醇溶解配置储存液（如200 mM），有效工作浓度为0.1-1 mM。
另有PMSF的无毒替代物，AEBSF（货号：20111ES50），为不可逆的丝氨酸蛋白酶抑制剂，活性和功能同PMSF，且易溶于水。
| 中文别名（Chinese synonym） | 苯甲磺酰氟；苯甲基黄酰氟；苯基甲基磺酰氟；苯甲基磺酰氟化物 |
| 英文别名（English synonym） | Phenylmethanesulfonyl fluoride; a-Toluenesulfonyl fluoride |
| CAS号（CAS NO.） | 329-98-6 |
| 分子式（Formula） | C7H7FO2S |
| 分子量（Molecular weight） | 174.2 |
| 外观（Appearance） | 白色至灰白色结晶 |
| 纯度（Purity） | ≥98.5% |
| 熔点（Melting point） | 91-94 °C |
| 溶解性（Solubility） | 难溶于水，溶于甲醇，乙醇，异丙醇等有机溶剂 |
| 结构式（Structure） |  |
常温运输。粉末2-8℃保存，2年有效。储存液4℃或-20℃分装保存，数月稳定。
Q：该产品对哪些蛋白酶有抑制效果？
A：苯甲基磺酰氟是一种非有机磷类的有机化合物，是一种广谱的酯酶/蛋白酶抑制剂， 可抑制丝氨酸蛋白酶，如胰蛋白酶(trypsin)和胰凝乳蛋白酶(chymotrypsin)；也可抑制半胱氨酸蛋白酶和乙酰胆碱酯酶的活性。同时苯甲基磺酰氟还可以与羰基化合物缩合制备烯烃。
Q：该产品通常在什么情况下使用？
A：PMSF 常在细胞或组织裂解之前加入到裂解液中以预防蛋白的降解。PMSF 可以单独使用，也常与其他蛋白酶抑制剂如胃抑肽酶 A 或者磷酸酶抑制剂如正钒酸钠混合使用。
Q：该产品主要应用在哪些实验？
A：PMSF 在药物代谢检测、植物蛋白提取等方面有着广泛的应用。
Q：该产品注意事项是什么？
A：PMSF 具有毒性和腐蚀性，对呼吸道黏膜、眼睛和皮肤有非常大的破坏性。可因吸入、咽下或皮肤吸收而致命。因此，使用时一定要做好个人防护，需佩戴防护手套和安全眼镜，并始终在化学通风橱内操作。同时，其对环境和水体可能存在危害，使用后还要注意做好危废处理。
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